Thinking outside the gate: single-cell assessments in multiple dimensions.

Pia Kvistborg,1,19 Cécile Gouttefangeas,2,19 Nima Aghaeepour,3 Angelica Cazaly,4 Pratip K. Chattopadhyay,5 Cliburn Chan,6 Judith Eckl,7 Greg Finak,8 Sine Reker Hadrup,9 Holden T. Maecker,10 Dominik Maurer,11 Tim Mosmann,12 Peng Qiu,13 Richard H. Scheuermann,14,15 Marij J.P. Welters,16 Guido Ferrari,17 Ryan R. Brinkman,3,20,* and Cedrik M. Britten18,20,21,* 1Netherlands Cancer Institute, 1006 BE Amsterdam, the Netherlands 2Institute for Cell Biology, Department of Immunology, University of Tübingen, 72076 Tuebingen, Germany 3Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC V5Z 1L3, Canada 4University of Southampton, Southampton SO17 1BJ, UK 5ImmunoTechnology Section, Vaccine Research Center, NIH, Bethesda, MD 20892, USA 6Department of Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC 27710, USA 7Helmholtz Zentrum München, 85764 Neuherberg, Germany 8Vaccine and Infectious Disease Division, Department of Biostatistics, Bioinformatics, and Epidemiology, Fred Hutchinson Cancer Research Center, Seattle, WA 98155, USA 9Center for Cancer Immune Therapy, Herlev Hospital, 2730 Herlev, Denmark 10Institute for Immunity, Transplantation, and Infection, Stanford School of Medicine, Stanford University, Stanford, CA 94305, USA 11Immatics Biotechnologies GmbH, 72076 Tübingen, Germany 12David D. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA 13Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, GA 30322, USA 14J. Craig Venter Institute, La Jolla, CA 92037, USA 15Department of Pathology, University of California, San Diego, San Diego, CA 92093, USA 16Department of Clinical Oncology, Leiden University Medical Center, 2300 RA Leiden, the Netherlands 17Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA 18BioNTech RNA Pharmaceuticals GmbH, 55131 Mainz, Germany 19Co-first author 20Co-senior author 21Present address: Immuno-Oncology & Combinations DPU, Oncology R&D, GSK, Stevenage SG1 2NY, UK *Correspondence: [email protected] (R.R.B.), [email protected] (C.M.B.) http://dx.doi.org/10.1016/j.immuni.2015.04.006